The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

Jesko Koehnke; Andrew F Bent; David Zollman; Kieran Smith; Wael E Houssen; Xiaofeng Zhu; Greg Mann; Tomas Lebl; Richard Scharff; Sally Shirran; Catherine H Botting; Marcel Jaspars; Ulrich Schwarz-Linek; James H Naismith

doi:10.1002/anie.201306302

The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

Jesko Koehnke, Andrew F Bent, David Zollman, Kieran Smith, Wael E Houssen, Xiaofeng Zhu, Greg Mann, Tomas Lebl, Richard Scharff, Sally Shirran, Catherine H Botting, Marcel Jaspars, Ulrich Schwarz-Linek, James H Naismith

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Abstract

Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

Original language	English
Pages (from-to)	13991-13996
Number of pages	6
Journal	Angewandte Chemie International Edition
Volume	52
Issue number	52
Early online date	8 Nov 2013
DOIs	https://doi.org/10.1002/anie.201306302
Publication status	Published - 23 Dec 2013

Bibliographical note

© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

adenylation
cyanobactins
heterocyclase
ribosomal peptide pathways

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Koehnke, J., Bent, A. F., Zollman, D., Smith, K., Houssen, W. E., Zhu, X., Mann, G., Lebl, T., Scharff, R., Shirran, S., Botting, C. H., Jaspars, M., Schwarz-Linek, U., & Naismith, J. H. (2013). The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction. Angewandte Chemie International Edition, 52(52), 13991-13996. https://doi.org/10.1002/anie.201306302

Koehnke, J, Bent, AF, Zollman, D, Smith, K, Houssen, WE, Zhu, X, Mann, G, Lebl, T, Scharff, R, Shirran, S, Botting, CH, Jaspars, M, Schwarz-Linek, U & Naismith, JH 2013, 'The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction', Angewandte Chemie International Edition, vol. 52, no. 52, pp. 13991-13996. https://doi.org/10.1002/anie.201306302

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abstract = "Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.",

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AU - Houssen, Wael E

AU - Zhu, Xiaofeng

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AU - Lebl, Tomas

AU - Scharff, Richard

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AU - Botting, Catherine H

AU - Jaspars, Marcel

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AU - Naismith, James H

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AB - Counting backwards: The cyanobactin class of heterocyclases, exemplified by TruD, possess an almost unique combination of processivity, specificity, chemical versatility, and promiscuity. TruD is shown by biochemical assay to be an adenylase, and processes cysteines in a defined order. The entire substrate leader can be removed and TruD will process a single specific cysteine residue; however the role of leader is to permit processivity through a balance of recognition. ATP/AMP=adenosine tri/monophosphate; PPi=pyrophosphate.

KW - adenylation

KW - cyanobactins

KW - heterocyclase

KW - ribosomal peptide pathways

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DO - 10.1002/anie.201306302

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IS - 52

ER -

The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction

Abstract

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Keywords

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