The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides

Wael E Houssen; Jesko Koehnke; David Zollman; Jeremie Vendome; Andrea Raab; Margaret C M Smith; James H Naismith; Marcel Jaspars

doi:10.1002/cbic.201200661

The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides

Wael E Houssen, Jesko Koehnke, David Zollman, Jeremie Vendome, Andrea Raab, Margaret C M Smith, James H Naismith, Marcel Jaspars

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Cyanobactins, including patellamides, are a group of cyanobacterial post-translationally modified ribosomal cyclic peptides. The final product should in theory be predictable from the sequence of the precursor peptide and the associated tailoring enzymes. Understanding the mechanism and recognition requirements of these enzymes will allow their rational engineering. We have identified three new cyanobactins from a Cyanothece PCC 7425 culture subjected to a heat shock. One of these compounds revealed a novel signature signal for ThcA, the subtilisin-like serine protease that is homologous to the patellamide protease PatA. The crystal structure of the latter and modelling studies allow rationalisation of the recognition determinants for both enzymes, consistent with the ribosomal biosynthetic origin of the new compounds.

Original language	English
Pages (from-to)	2683-2689
Number of pages	7
Journal	ChemBioChem
Volume	13
Issue number	18
Early online date	21 Nov 2012
DOIs	https://doi.org/10.1002/cbic.201200661
Publication status	Published - 21 Dec 2012

Bibliographical note

Acknowledgements
This work was supported by the Leverhulme Trust Grant RPG-20120504 (M.J., M.C.M.S. and J.H.N.). M.C.M.S. acknowledges funding from the BBSRC project grant BB/F003439/1. We thank DNA sequencing & Services (MRCPPU, College of Life Sciences,University of Dundee, Scotland, (www.dnaseq.co.uk) for DNA sequencing. W.E.H. thanks Drs. Louise Thomas and Andrew McEwan (University of Aberdeen) for help with protein purification. W.E.H. is the recipient of a SULSA postdoctoral fellowship. J.K. thanks Judith Reeks (University of St Andrews) for helpful discussion and Drs Catherine Botting and Sally Shirran (University of St Andrews) for MS analysis of cPatApr and PatE cleavage. J.K.is the recipient of a DFG postdoctoral fellowship.

Keywords

biosynthetic engineering
cyanobactins
natural products
ribosomal peptides
subtilisin proteases

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title = "The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides",

abstract = "Cyanobactins, including patellamides, are a group of cyanobacterial post-translationally modified ribosomal cyclic peptides. The final product should in theory be predictable from the sequence of the precursor peptide and the associated tailoring enzymes. Understanding the mechanism and recognition requirements of these enzymes will allow their rational engineering. We have identified three new cyanobactins from a Cyanothece PCC 7425 culture subjected to a heat shock. One of these compounds revealed a novel signature signal for ThcA, the subtilisin-like serine protease that is homologous to the patellamide protease PatA. The crystal structure of the latter and modelling studies allow rationalisation of the recognition determinants for both enzymes, consistent with the ribosomal biosynthetic origin of the new compounds.",

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author = "Houssen, {Wael E} and Jesko Koehnke and David Zollman and Jeremie Vendome and Andrea Raab and Smith, {Margaret C M} and Naismith, {James H} and Marcel Jaspars",

note = "Acknowledgements This work was supported by the Leverhulme Trust Grant RPG-20120504 (M.J., M.C.M.S. and J.H.N.). M.C.M.S. acknowledges funding from the BBSRC project grant BB/F003439/1. We thank DNA sequencing & Services (MRCPPU, College of Life Sciences,University of Dundee, Scotland, (www.dnaseq.co.uk) for DNA sequencing. W.E.H. thanks Drs. Louise Thomas and Andrew McEwan (University of Aberdeen) for help with protein purification. W.E.H. is the recipient of a SULSA postdoctoral fellowship. J.K. thanks Judith Reeks (University of St Andrews) for helpful discussion and Drs Catherine Botting and Sally Shirran (University of St Andrews) for MS analysis of cPatApr and PatE cleavage. J.K.is the recipient of a DFG postdoctoral fellowship.",

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AB - Cyanobactins, including patellamides, are a group of cyanobacterial post-translationally modified ribosomal cyclic peptides. The final product should in theory be predictable from the sequence of the precursor peptide and the associated tailoring enzymes. Understanding the mechanism and recognition requirements of these enzymes will allow their rational engineering. We have identified three new cyanobactins from a Cyanothece PCC 7425 culture subjected to a heat shock. One of these compounds revealed a novel signature signal for ThcA, the subtilisin-like serine protease that is homologous to the patellamide protease PatA. The crystal structure of the latter and modelling studies allow rationalisation of the recognition determinants for both enzymes, consistent with the ribosomal biosynthetic origin of the new compounds.

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The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides

Abstract

Bibliographical note

Keywords

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