Abstract
It is widely accepted that mechanical forces play a major role in lung development. We have previously shown that pulmonary hypoplasia caused by decreased fluid pressure in an Oligohydramnios murine model results in significant changes in the morphology of the lung spaces and the pulmonary epithelial cells. Here, we tested the effect of mechanical forces on the morphology of lung epithelial cells in vitro by applying cyclic stretch forces on cultured lung epithelial cells lines. Cells subjected to mechanical forces were compared to static cells. The cells were imaged and analyzed using different morphometric descriptors of size and shape. Our results show a significant change in the morphometry of lung epithelial cells in response to mechanical stress, where cells subjected to mechanical forces are larger and more regular than static cells as measured by fractal dimension. These findings add to the evidence that mechanical forces contribute to lung development and structure and suggest the usefulness of in vitro models for preclinical studies of lung biology.
Original language | English |
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Title of host publication | Proceedings - 2019 22nd International Conference on Control Systems and Computer Science, CSCS 2019 |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 521-524 |
Number of pages | 4 |
ISBN (Electronic) | 9781728123318 |
DOIs | |
Publication status | Published - May 2019 |
Event | 22nd International Conference on Control Systems and Computer Science, CSCS 2019 - Bucharest, Romania Duration: 28 May 2019 → 30 May 2019 |
Conference
Conference | 22nd International Conference on Control Systems and Computer Science, CSCS 2019 |
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Country/Territory | Romania |
City | Bucharest |
Period | 28/05/19 → 30/05/19 |
Bibliographical note
Funding Information:ACKNOWLEDGMENT This work was supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health, Number: P30GM114750; Department of Pediatrics; Kilguss Research Core of Women & Infants Hospital of Rhode Island.
Publisher Copyright:
© 2019 IEEE.
Keywords
- Cyclic stretch
- Fractal dimension
- Image analysis
- Lung epithelium
- Mechanical forces
- Morphometry