Mechanical forces play important roles in fetal lung development, therefore, pulmonary hypoplasia is an expected response to Oligohydramnios (OH) due to the decrease of fluid pressure. Amniotic sacs were punctured in pregnant mice with untouched fetuses serving as controls. Fetuses were delivered, and lung tissues collected. Histological sections were imaged and analyzed at the tissue level and the cellular level. Various morphometric descriptors of size, shape and differentiation were used to compare tissues from OH and untouched controls. Our results show that OH leads to smaller, less developed air spaces when compared to controls. Furthermore, cells from OH fetuses are smaller and less regular in shape compared to controls. There is also a defect in the differentiation of Type I alveolar epithelial cells. Here, we show a significant change in the morphometry of the fetal lung as a result of OH. These changes are consistent with the observed pulmonary hypoplasia and are important objective parameters that help in elucidating mechanisms of lung development and potential management of OH induced pulmonary hypoplasia.
|Title of host publication||2017 21st International Conference on Control Systems and Computer Science (CSCS)|
|Editors||Ioan Dumitrache, Adina Magda Florea, Florin Pop, Alexandru Dumitrașcu|
|Place of Publication||Bucharest, Romania|
|Number of pages||6|
|Publication status||Published - 7 Jul 2017|
|Event||21st International Conference on Control Systems and Computer Science (CSCS21) - Bucharest, Romania|
Duration: 29 May 2017 → 31 May 2017
|Conference||21st International Conference on Control Systems and Computer Science (CSCS21)|
|Period||29/05/17 → 31/05/17|
Bibliographical noteThis work was supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health, Number: P30GM114750; Department of Pediatrics; Kilguss Research Core of Women & Infants Hospital of Rhode Island.
- image analysis
- fractal dimension
- pulmonary hypoplasia