The effects of graded levels of calorie restriction: VIII. impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse

Sharon E Mitchell; Zhan Hui Tang; Celine Kerbois; Camille Delville; Davina Derous; Cara L Green; Yingchun Wang; Jackie J D Han; Luonan Chen; Alex Douglas; David Lusseau; Daniel E L Promislow; John R Speakman

doi:10.18632/oncotarget.15294

The effects of graded levels of calorie restriction: VIII. impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse

Sharon E Mitchell, Zhan Hui Tang, Celine Kerbois, Camille Delville, Davina Derous, Cara L Green, Yingchun Wang, Jackie J D Han, Luonan Chen, Alex Douglas, David Lusseau, Daniel E L Promislow, John R Speakman

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Abstract

Under calorie restriction (CR) animals need to lower energy demands. Whether this involves a reduction in cellular metabolism is an issue of contention. We exposed C57BL/6 mice to graded CR for 3 months, measured BMR and dissected out 20 body compartments. From a separate age-matched group (n=57), we built 7 predictive models for BMR. Unadjusted BMR declined with severity of restriction. Comparison of measured and predicted BMR from the simple models suggested suppression occurred. The extent of 'suppression' was greater with increased CR severity. However, when models based on individual organ sizes as predictors were used, the discrepancy between the prediction and the observed BMR disappeared. This suggested 'metabolic suppression' was an artefact of not having a detailed enough model to predict the expected changes in metabolism. Our data have wide implications because they indicate that inferred 'metabolic' impacts of genetic and other manipulations may reflect effects on organ morphology.

Original language	English
Pages (from-to)	17453-17474
Number of pages	22
Journal	Oncotarget
Volume	8
Issue number	11
Early online date	11 Feb 2017
DOIs	https://doi.org/10.18632/oncotarget.15294
Publication status	Published - 2017

Bibliographical note

We are grateful to the animal house staff for looking after the animals. The work was supported by the UK Biotechnology and Biological Sciences Research Council BBSRC (grants BB/G009953/1 and BB/J020028/1) to JRS and SEM. DD was supported by a studentship from the Centre for Genome Enabled Biology and Medicine, Aberdeen, UK, and CG was supported by a BBSRC EastBio studentship. Joint meetings were funded by a BBSRC China partnering award (BB/JO20028/1).

Keywords

metabolic rate
dietary restriction
protein restriction
calorie restriction
metabolic suppression
Gerotarget

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10.18632/oncotarget.15294Licence: CC BY

The effects of graded levels of calorie restriction: VIII. Impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse
https://creativecommons.org/licenses/by/4.0/
Final published version, 3.17 MBLicence: CC BY

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Mitchell, S. E., Tang, Z. H., Kerbois, C., Delville, C., Derous, D., Green, C. L., Wang, Y., Han, J. J. D., Chen, L., Douglas, A., Lusseau, D., Promislow, D. E. L., & Speakman, J. R. (2017). The effects of graded levels of calorie restriction: VIII. impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse. Oncotarget, 8(11), 17453-17474. https://doi.org/10.18632/oncotarget.15294

Mitchell, SE, Tang, ZH, Kerbois, C, Delville, C, Derous, D, Green, CL, Wang, Y, Han, JJD, Chen, L, Douglas, A , Lusseau, D, Promislow, DEL & Speakman, JR 2017, 'The effects of graded levels of calorie restriction: VIII. impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse', Oncotarget, vol. 8, no. 11, pp. 17453-17474. https://doi.org/10.18632/oncotarget.15294

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abstract = "Under calorie restriction (CR) animals need to lower energy demands. Whether this involves a reduction in cellular metabolism is an issue of contention. We exposed C57BL/6 mice to graded CR for 3 months, measured BMR and dissected out 20 body compartments. From a separate age-matched group (n=57), we built 7 predictive models for BMR. Unadjusted BMR declined with severity of restriction. Comparison of measured and predicted BMR from the simple models suggested suppression occurred. The extent of 'suppression' was greater with increased CR severity. However, when models based on individual organ sizes as predictors were used, the discrepancy between the prediction and the observed BMR disappeared. This suggested 'metabolic suppression' was an artefact of not having a detailed enough model to predict the expected changes in metabolism. Our data have wide implications because they indicate that inferred 'metabolic' impacts of genetic and other manipulations may reflect effects on organ morphology.",

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AB - Under calorie restriction (CR) animals need to lower energy demands. Whether this involves a reduction in cellular metabolism is an issue of contention. We exposed C57BL/6 mice to graded CR for 3 months, measured BMR and dissected out 20 body compartments. From a separate age-matched group (n=57), we built 7 predictive models for BMR. Unadjusted BMR declined with severity of restriction. Comparison of measured and predicted BMR from the simple models suggested suppression occurred. The extent of 'suppression' was greater with increased CR severity. However, when models based on individual organ sizes as predictors were used, the discrepancy between the prediction and the observed BMR disappeared. This suggested 'metabolic suppression' was an artefact of not having a detailed enough model to predict the expected changes in metabolism. Our data have wide implications because they indicate that inferred 'metabolic' impacts of genetic and other manipulations may reflect effects on organ morphology.

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KW - protein restriction

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KW - metabolic suppression

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The effects of graded levels of calorie restriction: VIII. impact of short term calorie and protein restriction on basal metabolic rate in the C57BL/6 mouse

Abstract

Bibliographical note

Keywords

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