The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant

Alexander Lorenz; Stephen C West; Matthew C Whitby

doi:10.1093/nar/gkp1179

The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant

Alexander Lorenz, Stephen C West, Matthew C Whitby

Medical Sciences

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Abstract

A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81δ strains results in Holliday junction resolution and crossover formation during meiosis.

Original language	English
Pages (from-to)	1866-1873
Number of pages	8
Journal	Nucleic Acids Research
Volume	38
Issue number	6
Early online date	29 Dec 2009
DOIs	https://doi.org/10.1093/nar/gkp1179
Publication status	Published - Apr 2010

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The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant

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