The PKC, HOG and Ca2+ signalling pathways co-ordinately regulate chitin synthesis in Candida albicans

Carol A Munro, Serena Selvaggini, Irene de Bruijn, Louise Walker, Megan D Lenardon, Bertus Gerssen, Sarah Milne, Alistair J P Brown, Neil A R Gow

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Chitin is an essential component of the fungal cell wall and its synthesis is under tight spatial and temporal regulation. The fungal human pathogen Candida albicans has a four member chitin synthase gene family comprising of CHS1 (class II), CHS2 (class I), CHS3 (class IV) and CHS8 (class I). LacZ reporters were fused to each CHS promoter to examine the transcriptional regulation of chitin synthesis. Each CHS promoter had a unique regulatory profile and responded to the addition of cell wall damaging agents, to mutations in specific CHS genes and exogenous Ca2+. The regulation of both CHS gene expression and chitin synthesis was co-ordinated by the PKC, HOG MAP kinase and Ca2+/calcineurin signalling pathways. Activation of these pathways also resulted in increased chitin synthase activity in vitro and elevated cell wall chitin content. Combinations of treatments that activated multiple pathways resulted in synergistic increases in CHS expression and in cell wall chitin content. Therefore, at least three pathways co-ordinately regulate chitin synthesis and activation of chitin synthesis operates at both transcriptional and post-transcriptional levels.

Original languageEnglish
Pages (from-to)1399-1413
Number of pages15
JournalMolecular Microbiology
Issue number5
Early online date8 Jan 2007
Publication statusPublished - Mar 2007

Bibliographical note

Open Access via PMC2649417


  • Artificial Gene Fusion
  • Calcium
  • Calcium Signaling
  • Candida albicans
  • Cell Wall
  • Chitin
  • Chitin Synthase
  • Gene Expression Regulation, Fungal
  • Genes, Reporter
  • Glycerol
  • Osmolar Concentration
  • Protein Kinase C
  • Signal Transduction
  • beta-Galactosidase


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