The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

Sylvie Janssens; Olaf van den Broekm; Ian R. Davenport; Robbert C. Akkers; Fei Liu; Gert Jan C. Veenstr; Stefan Hoppler; Kris Vleminckx; Olivier Destree

doi:10.1387/ijdb.120191kv

The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

Sylvie Janssens, Olaf van den Broekm, Ian R. Davenport, Robbert C. Akkers, Fei Liu, Gert Jan C. Veenstr, Stefan Hoppler, Kris Vleminckx, Olivier Destree^*

^*Corresponding author for this work

Medical Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.

Original language	English
Pages (from-to)	49-54
Number of pages	6
Journal	International Journal of Developmental Biology
Volume	57
Issue number	1
DOIs	https://doi.org/10.1387/ijdb.120191kv
Publication status	Published - 2013

Bibliographical note

We thank Dr. B. Gumbiner for providing the rabbit polyclonal antibody against Xenopus b-catenin and S.A. Blythe for sharing the Xenopus ChIP protocol. We thank Tim Deceunink,Thomas Roose and Yvonne Turnbull for animal care taking and Amin Bredan for editing the manuscript. O.v.d.B. and O.D. were supported by the IOP Genomics program (IGE01010), which is subsidized by the Dutch Ministry of Economic Affairs. We acknowledge additional support by the BBSRC (I.R.D. and S.H.) the AICR (F.L. and S.H.), the Netherlands Organization for Scientific Research, Earth and Life Sciences Council (NWO-ALW VIDI 864.03.002, R.C.A. and G.J.C.V.), the Research Foundation-Flanders, the Interuniversity Attraction Poles (IAP7/07), the Belgian Association against Cancer and the Concerted Research Actions (GOA) of Ghent University (S.J. and K.V.)

Keywords

foxd3
lefl/tcf
tcf7
Xenopus
Wnt
factor-binding sites
beta-catenin
differential expression
mesoderm development
distinct roles
transcription
genes
organizer
embryos
axis

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@article{26dc49806dbe4ed6836c05fb63848190,

title = "The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation",

abstract = "TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.",

keywords = "foxd3, lefl/tcf, tcf7, Xenopus, Wnt, factor-binding sites, beta-catenin, differential expression, mesoderm development, distinct roles, transcription, genes, organizer, embryos, axis",

author = "Sylvie Janssens and {van den Broekm}, Olaf and Davenport, {Ian R.} and Akkers, {Robbert C.} and Fei Liu and Veenstr, {Gert Jan C.} and Stefan Hoppler and Kris Vleminckx and Olivier Destree",

note = "We thank Dr. B. Gumbiner for providing the rabbit polyclonal antibody against Xenopus b-catenin and S.A. Blythe for sharing the Xenopus ChIP protocol. We thank Tim Deceunink,Thomas Roose and Yvonne Turnbull for animal care taking and Amin Bredan for editing the manuscript. O.v.d.B. and O.D. were supported by the IOP Genomics program (IGE01010), which is subsidized by the Dutch Ministry of Economic Affairs. We acknowledge additional support by the BBSRC (I.R.D. and S.H.) the AICR (F.L. and S.H.), the Netherlands Organization for Scientific Research, Earth and Life Sciences Council (NWO-ALW VIDI 864.03.002, R.C.A. and G.J.C.V.), the Research Foundation-Flanders, the Interuniversity Attraction Poles (IAP7/07), the Belgian Association against Cancer and the Concerted Research Actions (GOA) of Ghent University (S.J. and K.V.)",

year = "2013",

doi = "10.1387/ijdb.120191kv",

language = "English",

volume = "57",

pages = "49--54",

journal = "International Journal of Developmental Biology",

issn = "0214-6282",

publisher = "University of the Basque Country Press",

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T1 - The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

AU - Janssens, Sylvie

AU - van den Broekm, Olaf

AU - Davenport, Ian R.

AU - Akkers, Robbert C.

AU - Liu, Fei

AU - Veenstr, Gert Jan C.

AU - Hoppler, Stefan

AU - Vleminckx, Kris

AU - Destree, Olivier

N1 - We thank Dr. B. Gumbiner for providing the rabbit polyclonal antibody against Xenopus b-catenin and S.A. Blythe for sharing the Xenopus ChIP protocol. We thank Tim Deceunink,Thomas Roose and Yvonne Turnbull for animal care taking and Amin Bredan for editing the manuscript. O.v.d.B. and O.D. were supported by the IOP Genomics program (IGE01010), which is subsidized by the Dutch Ministry of Economic Affairs. We acknowledge additional support by the BBSRC (I.R.D. and S.H.) the AICR (F.L. and S.H.), the Netherlands Organization for Scientific Research, Earth and Life Sciences Council (NWO-ALW VIDI 864.03.002, R.C.A. and G.J.C.V.), the Research Foundation-Flanders, the Interuniversity Attraction Poles (IAP7/07), the Belgian Association against Cancer and the Concerted Research Actions (GOA) of Ghent University (S.J. and K.V.)

PY - 2013

Y1 - 2013

N2 - TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.

AB - TCF1 belongs to the family of LEF1/TCF transcription factors that regulate gene expression downstream of Wnt/beta-catenin signaling, which is crucial for embryonic development and is involved in adult stem cell regulation and tumor growth. In early Xenopus embryos, tcf1 plays an important role in mesoderm induction and patterning. Foxd3 emerged as a potential tcf1 target gene in a microarray analysis of gastrula stage embryos. Because foxd3 and tcf1 are coexpressed during gastrulation, we investigated whether foxd3 is regulated by tcf1. By using morpholino-mediated knockdown, we show that during gastrulation foxd3 expression is dependent on tcf1. By chromatin immunoprecipitation, we also demonstrate direct interaction of beta-catenin/tcf complexes with the foxd3 gene locus. Hence, our results indicate that tcf1 acts as an essential activator of foxd3, which is critical for dorsal mesoderm formation in early embryos.

KW - foxd3

KW - lefl/tcf

KW - tcf7

KW - Xenopus

KW - Wnt

KW - factor-binding sites

KW - beta-catenin

KW - differential expression

KW - mesoderm development

KW - distinct roles

KW - transcription

KW - genes

KW - organizer

KW - embryos

KW - axis

U2 - 10.1387/ijdb.120191kv

DO - 10.1387/ijdb.120191kv

M3 - Article

SN - 0214-6282

VL - 57

SP - 49

EP - 54

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

IS - 1

ER -

The Wnt signaling mediator tcf1 is required for expression of foxd3 during Xenopus gastrulation

Abstract

Bibliographical note

Keywords

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