Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans

Dev Kumar Verma; Luca Peruzza; Franziska Trusch; Manoj Kumar Yadav; null Ravindra; Sergei V. Shubin; Kenton L. Morgan; Vindhya Mohindra; Chris Hauton; Pieter van West; P. K. Pradhan; Neeraj Sood

doi:10.1016/j.ygeno.2020.10.028

Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans

Dev Kumar Verma, Luca Peruzza, Franziska Trusch, Manoj Kumar Yadav, Ravindra, Sergei V. Shubin, Kenton L. Morgan, Vindhya Mohindra, Chris Hauton, Pieter van West, P. K. Pradhan, Neeraj Sood^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.

Original language	English
Pages (from-to)	944-956
Number of pages	13
Journal	Genomics
Volume	113
Issue number	1P2
Early online date	28 Oct 2020
DOIs	https://doi.org/10.1016/j.ygeno.2020.10.028
Publication status	Published - 1 Jan 2021

Bibliographical note

Funding Information:
The work was carried out with financial support from Department of Biotechnology , Ministry of Science and Technology , Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16 ), and UK BBSRC , UK ESRC and UK Aid under contract BB/N005058/1 .

The authors would like to express their sincere thanks to Director, ICAR-NBFGR for providing facilities to carry out this study. The authors would also like to express their gratitude to Dr. Kuldeep K. Lal, Director, ICAR-NBFGR and Dr. J.K. Jena, DDG (Fy. Sc.), ICAR for their support, guidance and encouragement.

Keywords

Aphanomyces invadans
Common carp
Disease resistance
Head kidney
Immune response
RNA sequencing

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Verma, D. K., Peruzza, L., Trusch, F., Yadav, M. K., Ravindra, Shubin, S. V., Morgan, K. L., Mohindra, V., Hauton, C., van West, P., Pradhan, P. K., & Sood, N. (2021). Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans. Genomics, 113(1P2), 944-956. https://doi.org/10.1016/j.ygeno.2020.10.028

Verma, DK, Peruzza, L, Trusch, F, Yadav, MK, Ravindra, Shubin, SV, Morgan, KL, Mohindra, V, Hauton, C, van West, P, Pradhan, PK & Sood, N 2021, 'Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans', Genomics, vol. 113, no. 1P2, pp. 944-956. https://doi.org/10.1016/j.ygeno.2020.10.028

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abstract = "Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.",

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note = "Funding Information: The work was carried out with financial support from Department of Biotechnology , Ministry of Science and Technology , Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16 ), and UK BBSRC , UK ESRC and UK Aid under contract BB/N005058/1 . The authors would like to express their sincere thanks to Director, ICAR-NBFGR for providing facilities to carry out this study. The authors would also like to express their gratitude to Dr. Kuldeep K. Lal, Director, ICAR-NBFGR and Dr. J.K. Jena, DDG (Fy. Sc.), ICAR for their support, guidance and encouragement. ",

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AU - Peruzza, Luca

AU - Trusch, Franziska

AU - Yadav, Manoj Kumar

AU - Ravindra, null

AU - Shubin, Sergei V.

AU - Morgan, Kenton L.

AU - Mohindra, Vindhya

AU - Hauton, Chris

AU - van West, Pieter

AU - Pradhan, P. K.

AU - Sood, Neeraj

N1 - Funding Information: The work was carried out with financial support from Department of Biotechnology , Ministry of Science and Technology , Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015-16 ), and UK BBSRC , UK ESRC and UK Aid under contract BB/N005058/1 . The authors would like to express their sincere thanks to Director, ICAR-NBFGR for providing facilities to carry out this study. The authors would also like to express their gratitude to Dr. Kuldeep K. Lal, Director, ICAR-NBFGR and Dr. J.K. Jena, DDG (Fy. Sc.), ICAR for their support, guidance and encouragement.

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N2 - Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.

AB - Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.

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Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans

Abstract

Bibliographical note

Keywords

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