Abstract
The intestine, being a multifunctional organ central to both nutrient uptake, pathogen recognition and regulating the intestinal microbiome, has been subjected to intense research. This review will focus on the recent studies carried out using high-throughput gene expression approaches, such as microarray and RNA sequencing (RNA-seq). These techniques have advanced greatly in recent years, mainly as a result of the massive changes in sequencing methodologies. At the time of writing, there is a transition between relatively well characterised microarray platforms and the developing RNA-seq, with the prediction that within a few years as costs decrease and computation power increase, RNA-seq related approaches will supersede the microarrays. Comparisons between the approaches are made and specific examples of how the techniques have been used to examine intestinal responses to pathogens, dietary manipulations and osmoregulatory challenges are given.
Original language | English |
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Pages (from-to) | 103-117 |
Number of pages | 15 |
Journal | Developmental and Comparative Immunology |
Volume | 64 |
Early online date | 16 Mar 2016 |
DOIs | |
Publication status | Published - Nov 2016 |
Bibliographical note
AcknowledgementsThis study was funded by BBSRC grant BB/M026604/1, and UK Technology Strategy Board (TSB) grant 11974-81166. CED was funded by a BBSRC EastBio PhD studentship at University of Aberdeen. We are grateful to Chris Secombes, Helen Dooley and the two anonymous referees who made valuable comments on the earlier version of the manuscript.
Keywords
- microarray
- RNA-seq
- disease challenge
- oral vaccination
- gut inflammation
- osmoregulation