3D cell culture models of cancer are currently being developed to recapitulate in vivo physiological conditions and to assess therapeutic responses. However, most models failed to incorporate the biochemical and biophysical stimuli from fluid flow. In this study, a three-dimensional scaffold, SeedEZ was applied within the PerfusionPal perfused culture system to investigate how perfusion, and blood-like oxygen delivery influenced breast cancer cell growth and their responses to a commonly used breast cancer drug tamoxifen. Our results showed that breast cancer cells could be maintained over 3 weeks in PerfusionPal with increased cell viability compared to static 3D culture in fully humanised conditions. This platform also supported examining the effect of tamoxifen on breast cancer cell lines and in primary patient-derived breast cancer samples. Future work is warranted to further the adaption for fully humanised assessment of drug effectiveness in a patient personalized approach with the aim to reduce the burden of animal use in cancer research and increase the degree of human pre-clinical data translation to clinic.
Bibliographical noteOpen Access via the PLOS Agreement
Funding: This study was funded by NC3Rs studentship (NC/N00325X/1), NC3Rs/CRUK Skills and Capacity award (NC/T001259/1), the University of Aberdeen Development Trust, and the NIH SBIR Phase I and Phase II grants: 1R43TR001286, 2R44TR001286, and 5R44TR001286 awarded to Lena Biosciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.