Zinc Cluster Transcription Factors Alter Virulence in Candida albicans

Luca Issi, Rhys A. Farrer, Kelly Pastor, Benjamin Landry, Toni Delorey, George W Bell, Dawn A Thompson, Christina A Cuomo, Reeta P Rao (Corresponding Author)

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Almost all humans are colonized with Candida albicans However, in immunocompromised individuals, this benign commensal organism becomes a serious, life-threatening pathogen. Here, we describe and analyze the regulatory networks that modulate innate responses in the host niches. We identified Zcf15 and Zcf29, two Zinc Cluster transcription Factors (ZCF) that are required for C. albicans virulence. Previous sequence analysis of clinical C. albicans isolates from immunocompromised patients indicates that both ZCF genes diverged during clonal evolution. Using in vivo animal models, ex vivo cell culture methods, and in vitro sensitivity assays, we demonstrate that knockout mutants of both ZCF15 and ZCF29 are hypersensitive to reactive oxygen species (ROS), suggesting they help neutralize the host-derived ROS produced by phagocytes, as well as establish a sustained infection in vivo Transcriptomic analysis of mutants under resting conditions where cells were not experiencing oxidative stress revealed a large network that control macro and micronutrient homeostasis, which likely contributes to overall pathogen fitness in host niches. Under oxidative stress, both transcription factors regulate a separate set of genes involved in detoxification of ROS and down-regulating ribosome biogenesis. ChIP-seq analysis, which reveals vastly different binding partners for each transcription factor (TF) before and after oxidative stress, further confirms these results. Furthermore, the absence of a dominant binding motif likely facilitates their mobility, and supports the notion that they represent a recent expansion of the ZCF family in the pathogenic Candida species. Our analyses provide a framework for understanding new aspects of the interface between C. albicans and host defense response, and extends our understanding of how complex cell behaviors are linked to the evolution of TFs.

Original languageEnglish
Pages (from-to)559-576
Number of pages18
Issue number2
Early online date2 Feb 2017
Publication statusPublished - Feb 2017

Bibliographical note

The authors are grateful to A. Regev for her support on this project. We thank C. Ford and B. Haas and other members of the Regev laboratory for help with genomic data analysis and fruitful discussions; WPI undergraduate students, K. McCannel and M. Chiason for help on the in vivo genetic screening and L. Gaffney for her expertise on graphics of Figure 6. This project has been funded in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Grant Number U19AI110818 to the Broad Institute (C.A.C. and R.P.R.). R.A.F. is supported by the Wellcome Trust.


  • Candida Albicans
  • ZCF transcription factors
  • virulence
  • clonal evolution
  • gene duplication and expression
  • host interactions


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