Abstract
A gene whose predicted product shows 40-50% sequence identity with the products of nifS genes from nitrogen-fixing bacteria was found downstream from a cellulase gene in a DNA fragment from the cellulolytic rumen anaerobe, Ruminococus flavefaciens 17. The R. flavefaciens gene product released sulphur from L-cysteine when expressed in Escherichia coli, indicating that the R. flavefaciens NifS enzyme may play a role in sulphuration, perhaps, as in nitrogen-fixing bacteria, supplying sulphur to FeS proteins. Sequences hybridising with the R flavefaciens 17 nifS-like gene were also detected in R flavefaciens 007 and in R albus SY3. (C) 1998 Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 233-237 |
Number of pages | 5 |
Journal | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology |
Volume | 1386 |
Issue number | 1 |
Publication status | Published - 28 Jul 1998 |
Keywords
- cysteine desulfurase
- nifS
- rumen
- cellulolysis
- (Ruminococcus flavefaciens)
- AZOTOBACTER-VINELANDII
- ESCHERICHIA-COLI
- CLOSTRIDIUM-THERMOCELLUM
- RNASE-H
- NIFS
- CLUSTER
- CLONING
- NITROGENASES
- BIOSYNTHESIS
- CELLULOSOME