Activities per year
Abstract
The development of insulin resistance in the obese is associated with chronic, low-grade inflammation. We aimed to identify novel links between obesity, insulin resistance and the inflammatory response by comparing C57BL/6 with type I interleukin-1 receptor knockout (IL-1RI(-/-)) mice, which are protected against diet-induced insulin resistance. Mice were fed a high-fat diet for 16 wk. Insulin sensitivity was measured and proteomic analysis was performed on adipose, hepatic and skeletal muscle tissues. Despite an equal weight gain, IL-1RI(-/-) mice had lower plasma glucose, insulin and triacylglycerol concentrations, compared with controls, following dietary treatment. The higher insulin sensitivity in IL-1RI(-/-) mice was associated with down-regulation of antioxidant proteins and proteasomes in adipose tissue and hepatic soluble epoxide hydrolase, consistent with a compromised inflammatory response as well as increased glycolysis and decreased fatty acid beta-oxidation in their muscle. Their lower hepatic triacylglycerol concentrations may reflect decreased flux of free fatty acids to the liver, decreased hepatic fatty acid-binding protein expression and decreased lipogenesis. Correlation analysis revealed down-regulation of classical biomarkers of ER stress in their adipose tissue, suggesting that disruption of the IL-1RI-mediated inflammatory response may attenuate cellular stress, which was associated with significant protection from diet-induced insulin resistance, independent of obesity.
Original language | English |
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Pages (from-to) | 3244-3256 |
Number of pages | 13 |
Journal | Proteomics |
Volume | 9 |
Issue number | 12 |
DOIs | |
Publication status | Published - Jun 2009 |
Keywords
- adipose tissue
- animals
- chemokine CCL2
- epididymis
- epoxide hydrolases
- inflammation
- insulin resistance
- liver
- male
- membrane proteins
- mice
- mice, inbred C57BL
- mice, knockout
- mice, obese
- muscle, skeletal
- obesity
- principal component analysis
- proteome
- proteomics
- receptors, interleukin-1
- stress, physiological
- transcription factor RelA
- tumor necrosis factor-alpha
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Dive into the research topics of 'Attenuation of inflammation and cellular stress-related pathways maintains insulin sensitivity in obese type I interleukin-1 receptor knockout mice on a high-fat diet'. Together they form a unique fingerprint.Activities
- 1 Attending/organising a Conference
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NuGO week 2009
Vanessa Rungapamestry (Invited speaker)
2009Activity: Attending or organising an event › Attending/organising a Conference