Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Mark H.T. Stappers; Christina Nikolakopoulou; Darin L. Wiesner; Raif Yuecel; Bruce S. Klein; Janet A. Willment; Gordon D. Brown

doi:10.1002/eji.202149192

Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Mark H.T. Stappers, Christina Nikolakopoulou, Darin L. Wiesner, Raif Yuecel, Bruce S. Klein, Janet A. Willment, Gordon D. Brown^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.

Original language	English
Pages (from-to)	2341-2344
Number of pages	4
Journal	European Journal of Immunology
Volume	51
Issue number	9
Early online date	23 Jun 2021
DOIs	https://doi.org/10.1002/eji.202149192
Publication status	Published - Sept 2021

Bibliographical note

Funding Information:
We thank P. Asamaphan, A. Clark, and B. Kerscher for providing NIH overexpression cell lines, S. Yamasaki for the anti‐Mincle antibody, the staff of the University of Aberdeen animal facility for the care for our animals, and the Iain Fraser Cytometry Centre at the University of Aberdeen for their assistance. This work was supported by funding from the Wellcome Trust (102705, 217163), the Medical Research Council Centre for Medical Mycology, and the University of Exeter (MR/N006364/2).

Keywords

C-type lectin
Endothelial cells
Epithelial cells
Immunity, Innate

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10.1002/eji.202149192Licence: CC BY

Stappers_Etal_eji_Characterization_antifungal_Ctype_VOR
© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
Final published version, 1.21 MBLicence: CC BY

Cite this

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title = "Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues",

abstract = "Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues. ",

keywords = "C-type lectin, Endothelial cells, Epithelial cells, Immunity, Innate",

author = "Stappers, {Mark H.T.} and Christina Nikolakopoulou and Wiesner, {Darin L.} and Raif Yuecel and Klein, {Bruce S.} and Willment, {Janet A.} and Brown, {Gordon D.}",

note = "Funding Information: We thank P. Asamaphan, A. Clark, and B. Kerscher for providing NIH overexpression cell lines, S. Yamasaki for the anti‐Mincle antibody, the staff of the University of Aberdeen animal facility for the care for our animals, and the Iain Fraser Cytometry Centre at the University of Aberdeen for their assistance. This work was supported by funding from the Wellcome Trust (102705, 217163), the Medical Research Council Centre for Medical Mycology, and the University of Exeter (MR/N006364/2). ",

year = "2021",

month = sep,

doi = "10.1002/eji.202149192",

language = "English",

volume = "51",

pages = "2341--2344",

journal = "European Journal of Immunology",

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AU - Stappers, Mark H.T.

AU - Nikolakopoulou, Christina

AU - Wiesner, Darin L.

AU - Yuecel, Raif

AU - Klein, Bruce S.

AU - Willment, Janet A.

AU - Brown, Gordon D.

N1 - Funding Information: We thank P. Asamaphan, A. Clark, and B. Kerscher for providing NIH overexpression cell lines, S. Yamasaki for the anti‐Mincle antibody, the staff of the University of Aberdeen animal facility for the care for our animals, and the Iain Fraser Cytometry Centre at the University of Aberdeen for their assistance. This work was supported by funding from the Wellcome Trust (102705, 217163), the Medical Research Council Centre for Medical Mycology, and the University of Exeter (MR/N006364/2).

PY - 2021/9

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N2 - Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.

AB - Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.

KW - C-type lectin

KW - Endothelial cells

KW - Epithelial cells

KW - Immunity, Innate

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JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 9

ER -

Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Abstract

Bibliographical note

Keywords

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Iain Fraser Cytometry Centre

Cite this

Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Equipment

Iain Fraser Cytometry Centre

Cite this