Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severity

Reem Al-Rashoudi; Gillian Moir; Mohamed S. Al-Hajjaj; Monther M. Al-Alwan; Heather M. Wilson; Isabel J. Crane

doi:10.1186/s13223-019-0379-5

Differential expression of CCR2 and CX₃CR1 on CD16+ monocyte subsets is associated with asthma severity

Reem Al-Rashoudi, Gillian Moir, Mohamed S. Al-Hajjaj, Monther M. Al-Alwan, Heather M. Wilson, Isabel J. Crane^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

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Abstract

Background: Monocytes play an important role in immune and inflammatory diseases and monocyte subsets are predictors of disease in certain conditions. Expression of the chemokine receptors, CCR2 and CX3CR1 on monocyte subsets relates to their function and can be used in their characterization. Our objective was to determine whether CD14, CD16, CCR2 and CX3CR1 on monocyte subsets are potential indicators of asthma severity.

Methods: Blood samples were collected from Saudi Arabian patients with asthma and normal healthy individuals. Six-color flow-cytometry phenotypic analysis was used to identify human blood monocyte subsets, based on their expression of CD14 and CD16 following CD45 gating. Expression of CCR2 and CX3CR1 was analysed on classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) subsets and correlated with disease severity.

Results: We demonstrated a significant increase in percentage of total CD45-positive monocytes in the blood of patients with severe asthma, but the proportion of the individual monocyte subsets was not significantly changed when patients with mild, moderate and severe asthma were compared with healthy individuals. CD16 expression (Mean Fluorescence Intensity, MFI) was decreased on intermediate and non-classical subsets in patients with severe asthma compared to healthy controls. CX3CR1 expression was also lower, with a lower percentage of cells expressing CX3CR1 in the non-classical CD14+CD16++ subset in all patients with asthma and this was inversely related to the percentage of cells expressing CCR2.

Conclusions: CCR2 expression on monocytes indicated a tendency toward more phagocytic monocytes in patients with asthma. The differential expression of CD16, CX3CR1 and CCR2 on monocyte subsets in peripheral blood indicates modulation of the inflammatory response and suggests a role for monocytes in asthma pathogenesis.

Original language	English
Article number	64
Number of pages	11
Journal	Allergy, Asthma, and Clinical Immunology
Volume	15
DOIs	https://doi.org/10.1186/s13223-019-0379-5
Publication status	Published - 4 Nov 2019

Bibliographical note

Funding
Reem Al-Rashoudi was funded as part of the Saudi Arabian Cultural Bureau, External Joint Supervision Program (EJSP) to do a PhD degree jointly between Aberdeen University, UK and King Saud University, SA. The funding body played no part in the preparation of the data or the manuscript.

Acknowledgments
This work was supported by a grant from ‘Research Center, Center for Female Scientific and Medical Colleges, Deanship of Scientific Research, King Saud University. Support from the Saudi Arabian Cultural Bureau and External Joint Supervision Program (EJSP) is also gratefully acknowledged. Professor Graeme Devereux (Liverpool School of Tropical Medicine) provided helpful discussion on asthma. Dr. Raif Yuecel and Amer Al-Mazrou provided expertise for flow cytometry and analysis.

Keywords

Saudi Arabia
chemokine receptor
flow cytometry
CD14
biomarker
Biomarker
Chemokine receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Al-Rashoudi2019_Article_DifferentialExpressionOfCCR2An_VOR
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Cite this

@article{a83b52e196d743dd9e02c8d841fd2f0e,

title = "Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severity",

abstract = "Background: Monocytes play an important role in immune and inflammatory diseases and monocyte subsets are predictors of disease in certain conditions. Expression of the chemokine receptors, CCR2 and CX3CR1 on monocyte subsets relates to their function and can be used in their characterization. Our objective was to determine whether CD14, CD16, CCR2 and CX3CR1 on monocyte subsets are potential indicators of asthma severity.Methods: Blood samples were collected from Saudi Arabian patients with asthma and normal healthy individuals. Six-color flow-cytometry phenotypic analysis was used to identify human blood monocyte subsets, based on their expression of CD14 and CD16 following CD45 gating. Expression of CCR2 and CX3CR1 was analysed on classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) subsets and correlated with disease severity.Results: We demonstrated a significant increase in percentage of total CD45-positive monocytes in the blood of patients with severe asthma, but the proportion of the individual monocyte subsets was not significantly changed when patients with mild, moderate and severe asthma were compared with healthy individuals. CD16 expression (Mean Fluorescence Intensity, MFI) was decreased on intermediate and non-classical subsets in patients with severe asthma compared to healthy controls. CX3CR1 expression was also lower, with a lower percentage of cells expressing CX3CR1 in the non-classical CD14+CD16++ subset in all patients with asthma and this was inversely related to the percentage of cells expressing CCR2.Conclusions: CCR2 expression on monocytes indicated a tendency toward more phagocytic monocytes in patients with asthma. The differential expression of CD16, CX3CR1 and CCR2 on monocyte subsets in peripheral blood indicates modulation of the inflammatory response and suggests a role for monocytes in asthma pathogenesis.",

keywords = "Saudi Arabia, chemokine receptor, flow cytometry, CD14, biomarker, Biomarker, Chemokine receptor",

author = "Reem Al-Rashoudi and Gillian Moir and Al-Hajjaj, {Mohamed S.} and Al-Alwan, {Monther M.} and Wilson, {Heather M.} and Crane, {Isabel J.}",

note = "Funding Reem Al-Rashoudi was funded as part of the Saudi Arabian Cultural Bureau, External Joint Supervision Program (EJSP) to do a PhD degree jointly between Aberdeen University, UK and King Saud University, SA. The funding body played no part in the preparation of the data or the manuscript. Acknowledgments This work was supported by a grant from {\textquoteleft}Research Center, Center for Female Scientific and Medical Colleges, Deanship of Scientific Research, King Saud University. Support from the Saudi Arabian Cultural Bureau and External Joint Supervision Program (EJSP) is also gratefully acknowledged. Professor Graeme Devereux (Liverpool School of Tropical Medicine) provided helpful discussion on asthma. Dr. Raif Yuecel and Amer Al-Mazrou provided expertise for flow cytometry and analysis. ",

year = "2019",

month = nov,

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doi = "10.1186/s13223-019-0379-5",

language = "English",

volume = "15",

journal = "Allergy, Asthma, and Clinical Immunology",

issn = "1710-1484",

publisher = "BioMed Central",

}

TY - JOUR

T1 - Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severity

AU - Al-Rashoudi, Reem

AU - Moir, Gillian

AU - Al-Hajjaj, Mohamed S.

AU - Al-Alwan, Monther M.

AU - Wilson, Heather M.

AU - Crane, Isabel J.

N1 - Funding Reem Al-Rashoudi was funded as part of the Saudi Arabian Cultural Bureau, External Joint Supervision Program (EJSP) to do a PhD degree jointly between Aberdeen University, UK and King Saud University, SA. The funding body played no part in the preparation of the data or the manuscript. Acknowledgments This work was supported by a grant from ‘Research Center, Center for Female Scientific and Medical Colleges, Deanship of Scientific Research, King Saud University. Support from the Saudi Arabian Cultural Bureau and External Joint Supervision Program (EJSP) is also gratefully acknowledged. Professor Graeme Devereux (Liverpool School of Tropical Medicine) provided helpful discussion on asthma. Dr. Raif Yuecel and Amer Al-Mazrou provided expertise for flow cytometry and analysis.

PY - 2019/11/4

Y1 - 2019/11/4

N2 - Background: Monocytes play an important role in immune and inflammatory diseases and monocyte subsets are predictors of disease in certain conditions. Expression of the chemokine receptors, CCR2 and CX3CR1 on monocyte subsets relates to their function and can be used in their characterization. Our objective was to determine whether CD14, CD16, CCR2 and CX3CR1 on monocyte subsets are potential indicators of asthma severity.Methods: Blood samples were collected from Saudi Arabian patients with asthma and normal healthy individuals. Six-color flow-cytometry phenotypic analysis was used to identify human blood monocyte subsets, based on their expression of CD14 and CD16 following CD45 gating. Expression of CCR2 and CX3CR1 was analysed on classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) subsets and correlated with disease severity.Results: We demonstrated a significant increase in percentage of total CD45-positive monocytes in the blood of patients with severe asthma, but the proportion of the individual monocyte subsets was not significantly changed when patients with mild, moderate and severe asthma were compared with healthy individuals. CD16 expression (Mean Fluorescence Intensity, MFI) was decreased on intermediate and non-classical subsets in patients with severe asthma compared to healthy controls. CX3CR1 expression was also lower, with a lower percentage of cells expressing CX3CR1 in the non-classical CD14+CD16++ subset in all patients with asthma and this was inversely related to the percentage of cells expressing CCR2.Conclusions: CCR2 expression on monocytes indicated a tendency toward more phagocytic monocytes in patients with asthma. The differential expression of CD16, CX3CR1 and CCR2 on monocyte subsets in peripheral blood indicates modulation of the inflammatory response and suggests a role for monocytes in asthma pathogenesis.

AB - Background: Monocytes play an important role in immune and inflammatory diseases and monocyte subsets are predictors of disease in certain conditions. Expression of the chemokine receptors, CCR2 and CX3CR1 on monocyte subsets relates to their function and can be used in their characterization. Our objective was to determine whether CD14, CD16, CCR2 and CX3CR1 on monocyte subsets are potential indicators of asthma severity.Methods: Blood samples were collected from Saudi Arabian patients with asthma and normal healthy individuals. Six-color flow-cytometry phenotypic analysis was used to identify human blood monocyte subsets, based on their expression of CD14 and CD16 following CD45 gating. Expression of CCR2 and CX3CR1 was analysed on classical (CD14++CD16-), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) subsets and correlated with disease severity.Results: We demonstrated a significant increase in percentage of total CD45-positive monocytes in the blood of patients with severe asthma, but the proportion of the individual monocyte subsets was not significantly changed when patients with mild, moderate and severe asthma were compared with healthy individuals. CD16 expression (Mean Fluorescence Intensity, MFI) was decreased on intermediate and non-classical subsets in patients with severe asthma compared to healthy controls. CX3CR1 expression was also lower, with a lower percentage of cells expressing CX3CR1 in the non-classical CD14+CD16++ subset in all patients with asthma and this was inversely related to the percentage of cells expressing CCR2.Conclusions: CCR2 expression on monocytes indicated a tendency toward more phagocytic monocytes in patients with asthma. The differential expression of CD16, CX3CR1 and CCR2 on monocyte subsets in peripheral blood indicates modulation of the inflammatory response and suggests a role for monocytes in asthma pathogenesis.

KW - Saudi Arabia

KW - chemokine receptor

KW - flow cytometry

KW - CD14

KW - biomarker

KW - Biomarker

KW - Chemokine receptor

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U2 - 10.1186/s13223-019-0379-5

DO - 10.1186/s13223-019-0379-5

M3 - Article

C2 - 31700522

SN - 1710-1484

VL - 15

JO - Allergy, Asthma, and Clinical Immunology

JF - Allergy, Asthma, and Clinical Immunology

M1 - 64

ER -

Differential expression of CCR2 and CX₃CR1 on CD16+ monocyte subsets is associated with asthma severity

Abstract

Bibliographical note

Keywords

UN SDGs

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Heather Wilson

Iain Fraser Cytometry Centre

Cite this

Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severity

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Profiles

Heather Wilson

Equipment

Iain Fraser Cytometry Centre

Cite this

Differential expression of CCR2 and CX₃CR1 on CD16+ monocyte subsets is associated with asthma severity