Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis

Oriana Rossi; M Tanweer Khan; Martin Schwarzer; Tomas Hudcovic; Dagmar Srutkova; Sylvia H Duncan; Ellen H Stolte; Hana Kozakova; Harry J Flint; Janneke N Samsom; Hermie J M Harmsen; Jerry M Wells

doi:10.1371/journal.pone.0123013

Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis

Oriana Rossi, M Tanweer Khan, Martin Schwarzer, Tomas Hudcovic, Dagmar Srutkova, Sylvia H Duncan, Ellen H Stolte, Hana Kozakova, Harry J Flint, Janneke N Samsom, Hermie J M Harmsen, Jerry M Wells

The Rowett Institute of Nutrition and Health

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Abstract

A decrease in the abundance and biodiversity of intestinal bacteria within the Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, the anti-inflammatory bacterium Faecalibacterium prausnitzii, member of the Firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of IBD patients. The aim of this study was to investigate the immunomodulatory properties of F. prausnitzii strain A2-165, the biofilm forming strain HTF-F and the extracellular polymeric matrix (EPM) isolated from strain HTF-F. For this purpose, the immunomodulatory properties of the F. prausnitzii strains and the EPM were studied in vitro using human monocyte-derived dendritic cells. Then, the capacity of the F. prausnitzii strains and the EPM of HTF-F to suppress inflammation was assessed in vivo in the mouse dextran sodium sulphate (DSS) colitis model. The F. prausnitzii strains and the EPM had anti-inflammatory effects on the clinical parameters measured in the DSS model but with different efficacy. The immunomodulatory effects of the EPM were mediated through the TLR2-dependent modulation of IL-12 and IL-10 cytokine production in antigen presenting cells, suggesting that it contributes to the anti-inflammatory potency of F. prausnitzii HTF-F. The results show that F. prausnitzii HTF-F and its EPM may have a therapeutic use in IBD.

Original language	English
Article number	e0123013
Number of pages	17
Journal	PloS ONE
Volume	10
Issue number	4
Early online date	24 Apr 2015
DOIs	https://doi.org/10.1371/journal.pone.0123013
Publication status	Published - 24 Apr 2015

Bibliographical note

Date of Acceptance: 26/02/2015

Funding: The authors received no specific funding for this work.

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Rossi, O., Khan, M. T., Schwarzer, M., Hudcovic, T., Srutkova, D., Duncan, S. H., Stolte, E. H., Kozakova, H., Flint, H. J., Samsom, J. N., Harmsen, H. J. M., & Wells, J. M. (2015). Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis. PloS ONE, 10(4), Article e0123013. https://doi.org/10.1371/journal.pone.0123013

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AU - Khan, M Tanweer

AU - Schwarzer, Martin

AU - Hudcovic, Tomas

AU - Srutkova, Dagmar

AU - Duncan, Sylvia H

AU - Stolte, Ellen H

AU - Kozakova, Hana

AU - Flint, Harry J

AU - Samsom, Janneke N

AU - Harmsen, Hermie J M

AU - Wells, Jerry M

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AB - A decrease in the abundance and biodiversity of intestinal bacteria within the Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, the anti-inflammatory bacterium Faecalibacterium prausnitzii, member of the Firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of IBD patients. The aim of this study was to investigate the immunomodulatory properties of F. prausnitzii strain A2-165, the biofilm forming strain HTF-F and the extracellular polymeric matrix (EPM) isolated from strain HTF-F. For this purpose, the immunomodulatory properties of the F. prausnitzii strains and the EPM were studied in vitro using human monocyte-derived dendritic cells. Then, the capacity of the F. prausnitzii strains and the EPM of HTF-F to suppress inflammation was assessed in vivo in the mouse dextran sodium sulphate (DSS) colitis model. The F. prausnitzii strains and the EPM had anti-inflammatory effects on the clinical parameters measured in the DSS model but with different efficacy. The immunomodulatory effects of the EPM were mediated through the TLR2-dependent modulation of IL-12 and IL-10 cytokine production in antigen presenting cells, suggesting that it contributes to the anti-inflammatory potency of F. prausnitzii HTF-F. The results show that F. prausnitzii HTF-F and its EPM may have a therapeutic use in IBD.

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Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis

Abstract

Bibliographical note

UN SDGs

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