Functional mapping of yeast genomes by saturated transposition

Agnès H Michel, Riko Hatakeyama, Philipp Kimmig, Meret Arter, Matthias Peter, Joao Matos, Claudio De Virgilio, Benoît Kornmann* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)
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Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput.

Original languageEnglish
Article numbere23570
Number of pages28
Early online date8 May 2017
Publication statusPublished - 9 Jun 2017

Bibliographical note

We thank Beat Christen for inspiring this work and expert insight on Tn-seq, Reinhard Kunze for kind gift of plasmid and strain, Asun Monfort Pineda and Anton Wutz for invaluable help with Illumina Sequencing, Jeremy Thorner, Karsten Weis, Jeffrey Tang, Judith Berman and Vladimir Gritsenko for helpful discussions, Christine Doderer for preliminary experiments, Alicia Smith for comments on the manuscript, and the Kornmann lab for comments and ideas. This work is supported by grants of the Swiss National Science Foundation (PP00P3_13365 to BK, 310030_166474 to CDV, 31003A_153058 and 155823 to JM), the European Research Council (337906-OrgaNet) to BK, and PK is supported by the Human Frontier Science Program Organization


  • DNA Transposable Elements
  • Genes, Fungal
  • Genetics, Microbial/methods
  • Genome, Fungal
  • Molecular Sequence Annotation/methods
  • Mutagenesis, Insertional/methods
  • Saccharomyces cerevisiae/genetics
  • Sequence Analysis, DNA


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