Genome size variation in deep-sea amphipods

Heather Ritchie; Alan John Jamieson; Stuart Brannon Piertney

doi:10.1098/rsos.170862

Genome size variation in deep-sea amphipods

Heather Ritchie, Alan John Jamieson, Stuart Brannon Piertney

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

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Abstract

Genome size varies considerably across taxa, and extensive research effort has gone into understanding whether variation can be explained by differences in key ecological and life-history traits among species. The extreme environmental conditions that characterize the deep sea have been hypothesized to promote large genome sizes in eukaryotes. Here we test this supposition by examining genome sizes among 13 species of deep-sea amphipods from the Mariana, Kermadec and New Hebrides trenches. Genome sizes were estimated using flow cytometry and found to vary nine-fold, ranging from 4.06 pg (4.04 Gb) in Paralicella caperesca to 34.79 pg (34.02 Gb) in Alicella gigantea. Phylogenetic independent contrast analysis identified a relationship between genome size and maximum body size, though this was largely driven by those species that display size gigantism. There was a distinct shift in the genome size trait diversification rate in the supergiant amphipod A. gigantea relative to the rest of the group. The variation in genome size observed is striking and argues against genome size being driven by a common evolutionary history, ecological niche and life-history strategy in deep-sea amphipods.

Original language	English
Article number	170862
Pages (from-to)	1-9
Number of pages	9
Journal	Royal Society Open Science
Volume	4
Early online date	13 Sept 2017
DOIs	https://doi.org/10.1098/rsos.170862
Publication status	Published - Sept 2017

Bibliographical note

Funding: This work was supported by the HADEEP projects, funded by the Nippon Foundation, Japan (2009765188); the Natural Environment Research Council (NERC), UK (NE/E007171/1); Total Foundation, France; National Institute of Water and Atmospheric Research (NIWA), New Zealand (CO1_0906); Schmidt Ocean Institute, USA (FK141109) (A.J.J. and S.B.P); Marine Alliance for Science and Technology for Scotland (MASTS) (HR09011 and DSSG15) (H.R., A.J.J., S.B.P); and the Leverhulme Trust (S.B.P.).

Acknowledgements: We thank the chief scientists, crew and company of the New Zealand RV Kaharoa (KAH1301 and KAH1310) and the United States RV Falkor (Cruise FK141109). From NIWA, we thank Malcolm Clark, Ashley Rowden, Kareen Schnabel, and Sadie Mills for logistical support at the NIWA Invertebrate Collection. We thank NOAA Marine National Monuments, Richard Hall and Eric Breuer for their support and collaboration. We also thank Attila Bebes and the Iain Fraser Cytometry Centre (IFCC) for technical assistance.

Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3868216.

Keywords

genome size
deep sea
amphipods
gigantism
adaptation
evolution

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10.1098/rsos.170862Licence: CC BY

Genome size variation in deep-sea amphipods
© 2017 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Final published version, 574 KBLicence: CC BY

Genome size variation in deep-sea amphipods.
Piertney, S. (Creator), Ritchie, H. (Creator) & Jamieson, A. J. (Creator), Figshare, 8 Sept 2017
DOI: 10.6084/m9.figshare.c.3868216.v2
Dataset

Iain Fraser Cytometry Centre
Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)
Institute of Medical Sciences
Research Facilities: Facility

Cite this

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abstract = "Genome size varies considerably across taxa, and extensive research effort has gone into understanding whether variation can be explained by differences in key ecological and life-history traits among species. The extreme environmental conditions that characterize the deep sea have been hypothesized to promote large genome sizes in eukaryotes. Here we test this supposition by examining genome sizes among 13 species of deep-sea amphipods from the Mariana, Kermadec and New Hebrides trenches. Genome sizes were estimated using flow cytometry and found to vary nine-fold, ranging from 4.06 pg (4.04 Gb) in Paralicella caperesca to 34.79 pg (34.02 Gb) in Alicella gigantea. Phylogenetic independent contrast analysis identified a relationship between genome size and maximum body size, though this was largely driven by those species that display size gigantism. There was a distinct shift in the genome size trait diversification rate in the supergiant amphipod A. gigantea relative to the rest of the group. The variation in genome size observed is striking and argues against genome size being driven by a common evolutionary history, ecological niche and life-history strategy in deep-sea amphipods.",

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note = "Funding: This work was supported by the HADEEP projects, funded by the Nippon Foundation, Japan (2009765188); the Natural Environment Research Council (NERC), UK (NE/E007171/1); Total Foundation, France; National Institute of Water and Atmospheric Research (NIWA), New Zealand (CO1_0906); Schmidt Ocean Institute, USA (FK141109) (A.J.J. and S.B.P); Marine Alliance for Science and Technology for Scotland (MASTS) (HR09011 and DSSG15) (H.R., A.J.J., S.B.P); and the Leverhulme Trust (S.B.P.). Acknowledgements: We thank the chief scientists, crew and company of the New Zealand RV Kaharoa (KAH1301 and KAH1310) and the United States RV Falkor (Cruise FK141109). From NIWA, we thank Malcolm Clark, Ashley Rowden, Kareen Schnabel, and Sadie Mills for logistical support at the NIWA Invertebrate Collection. We thank NOAA Marine National Monuments, Richard Hall and Eric Breuer for their support and collaboration. We also thank Attila Bebes and the Iain Fraser Cytometry Centre (IFCC) for technical assistance. Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3868216.",

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N2 - Genome size varies considerably across taxa, and extensive research effort has gone into understanding whether variation can be explained by differences in key ecological and life-history traits among species. The extreme environmental conditions that characterize the deep sea have been hypothesized to promote large genome sizes in eukaryotes. Here we test this supposition by examining genome sizes among 13 species of deep-sea amphipods from the Mariana, Kermadec and New Hebrides trenches. Genome sizes were estimated using flow cytometry and found to vary nine-fold, ranging from 4.06 pg (4.04 Gb) in Paralicella caperesca to 34.79 pg (34.02 Gb) in Alicella gigantea. Phylogenetic independent contrast analysis identified a relationship between genome size and maximum body size, though this was largely driven by those species that display size gigantism. There was a distinct shift in the genome size trait diversification rate in the supergiant amphipod A. gigantea relative to the rest of the group. The variation in genome size observed is striking and argues against genome size being driven by a common evolutionary history, ecological niche and life-history strategy in deep-sea amphipods.

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Genome size variation in deep-sea amphipods

Abstract

Bibliographical note

Keywords

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Datasets

Genome size variation in deep-sea amphipods.

Equipment

Iain Fraser Cytometry Centre

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