Abstract
With increased longevity and subsequent rise in people with age-related neurodegenerative diseases, protection of neurons from oxidative stress damage has become an important field of study. For the first time, we highlight the neuroprotective properties of rapeseed pomace (RSP) extract in SH-SY5Y human neuroblastoma cells. We used resazurin to determine cell metabolism, 2,7’-dichlorofluorescin diacetate (H2DCFDA) to assess the potential of RSP extracts to shield cells from reactive oxygen species (ROS) induced by H2O2 using flow cytometry, HPLC to analyze for malondialdehyde (MDA) as a lipid peroxidation marker and the COMET assay to assess DNA strand breakage. Protein stress arrays were used to investigate the cellular pathways affected by RSP extract. No effect on cell metabolism in SH-SY5Y cells was observed after RSP extract treatment (up to 1.5 mg/ml). Pretreatment (24 hr) with RSP extract (1 mg/ml), before H2O2-induced stress, alleviated ROS production and DNA strand breakage by 68%, and 38%, respectively. At protein level, the RSP extract increased the levels of FABP-1, HIF-1α, SOD2, and Cytochrome c proteins. Under H2O2-induced stress, however, it helped to downregulate p38α levels, a protein kinase which is receptive to stress impulse (mitogen-activated). RSP extract shows very promising cell protective properties in relation to oxidative stress. Practical applications: Oxidative stress has been associated with numerous diseases for example cancer, diabetes, and many neurological disorders including Parkinson's and Alzheimer's diseases. Hence, there is acceptance among the scientific community of antioxidant therapy and the quest for effective, low cost and readily available sources of natural antioxidants is paramount. Rapeseed plantations are abundant around the world due to the use of rapeseed oil in cooking and as a biofuel. The resulting rapeseed pomace (by-product), specifically its extract, contains high levels of phytochemicals that protect cells against oxidative stress. Therefore, RSP extract can potentially be used/developed as functional food and nutraceuticals in the prevention of many complex neurodegenerative diseases.
| Original language | English |
|---|---|
| Article number | e13592 |
| Number of pages | 10 |
| Journal | Journal of Food Biochemistry |
| Volume | 45 |
| Issue number | 2 |
| Early online date | 22 Dec 2020 |
| DOIs | |
| Publication status | Published - 22 Feb 2021 |
Bibliographical note
Funding Information:Thanks is given to Macintosh of Glendaveny for supplying the rapeseed pomace samples for this study; Professor Susan Duthie and Dr. Elena Lendoiro for their advice and guidance on the comet assay; Ruben Van Gestel for his technical support and Tenovus Scotland for financing this project.
Data Availability Statement
Data available in article Supporting Information.Keywords
- comet assay
- cytoprotection
- plant extract
- rapeseed
- ROS
- SH-SY5Y neuroblastoma cells
Access to Document
- Pohl_etal_JFB_Impact_Of_Rapeseed_AAM
This is the peer reviewed version of the following article: Pohl F, Goua M, Yates K, et al. Impactof rapeseed pomace extract on markers of oxidative stress and DNA damage in human SH-SY5Y cells. J Food Biochem. 2021;45:e13592. https://doi.org/10.1111/jfbc.13592, which has been published in final form at https://doi.org/10.1111/jfbc.13592. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.