Oral treatment with Eubacterium hallii improves insulin sensitivity in db/db mice

Shanthadevi Udayappan, Louise Manneras-Holm, Alice Chaplin-Scott, Clara Belzer, Hilde Herrema, Geesje M Dallinga-Thie, Erik S G Stroes, Albert K Groen, Harry J Flint, Fredrik Backhed, Willem M de Vos, Max Nieuwdorp, Sylvia Helen Duncan

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)
7 Downloads (Pure)


An altered intestinal microbiota composition is associated with insulin resistance and type 2 diabetes mellitus. We previously identified increased intestinal levels of Eubacterium hallii, an anaerobic bacterium belonging to the butyrate-producing Lachnospiraceae family, in metabolic syndrome subjects who received a faecal transplant from a lean donor. To further assess the effects of E. hallii on insulin sensitivity, we orally treated obese and diabetic db/db mice with alive E. hallii and glycerol or heat-inactive E. hallii as control. Insulin tolerance tests and hyperinsulinemic-euglycemic clamp experiments revealed that alive E. hallii treatment improved insulin sensitivity compared control treatment. In addition, E. hallii treatment increased energy expenditure in db/db mice. Active E. hallii treatment was found to increase faecal butyrate concentrations and to modify bile acid metabolism compared with heat-inactivated controls. Our data suggest that E. hallii administration potentially alters the function of the intestinal microbiome and that microbial metabolites may contribute to the improved metabolic phenotype.

Original languageEnglish
Article number16009
JournalNPJ biofilms and microbiomes
Publication statusPublished - 6 Jul 2016

Bibliographical note

F.B. is supported by Swedish Research Council, Swedish Diabetes Foundation, Swedish Heart Lung Foundation, Swedish Foundation for Strategic Research, Knut and Alice Wallenberg foundation, Göran Gustafsson Foundation, Ingbritt and Arne Lundberg’s foundation, Swedish Heart Lung Foundation, Torsten Söderberg’s Foundation, Ragnar Söderberg’s Foundation, NovoNordisk Foundation, AFA insurances, and LUA-ALF grants from Västra Götalandsregionen and Stockholm County Council. F.B. is a recipient of ERC Consolidator Grant (European Research Council, Consolidator grant 615362—METABASE). W.M.d.V. is supported by the Finland Academy of Sciences (grants 137389, 141140 and 1272870 ), the Netherlands Organization for Scientific Research (Spinoza Award and SIAM Gravity Grant 024.002.002) and the European Research Council (ERC Advanced Grant 250172 MicrobesInside). M.N. is supported by a ZONMW-VIDI grant 2013 (016.146.327).


  • Journal Article


Dive into the research topics of 'Oral treatment with Eubacterium hallii improves insulin sensitivity in db/db mice'. Together they form a unique fingerprint.

Cite this