Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1

Madhusudhan  Srinivasan; Naomi J  Petela; Johanna C  Scheinost; James  Collier; Menelaos  Voulgaris; Maurici B  Roig; Frederic  Beckouët; Bin Hu; Kim A  Nasmyth

doi:10.7554/elife.44736

Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1

Madhusudhan Srinivasan^* (Corresponding Author), Naomi J Petela, Johanna C Scheinost, James Collier, Menelaos Voulgaris, Maurici B Roig, Frederic Beckouët, Bin Hu, Kim A Nasmyth

^*Corresponding author for this work

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Abstract

Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.

Original language	English
Article number	e44736
Number of pages	34
Journal	eLife
Volume	8
Early online date	21 Jun 2019
DOIs	https://doi.org/10.7554/elife.44736
Publication status	Published - 21 Jun 2019
Externally published	Yes

Bibliographical note

Acknowledgements
Maria Demidova conducted initial experiments that this study expanded on. We are grateful to Tomo Tanaka and Seiji Tanaka for supplying reagents. We thank all members of the Nasmyth group for valuable discussions, technical assistance and critical reading of the manuscript. This work was funded by the Wellcome Trust Senior Investigator Award, Grant Ref 107935/Z/15/Z and Cancer Research UK Programme Grant, Grant Ref 26747 to KN. BH is funded by (202062/Z/16/Z).

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Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1
Srinivasan, M. (Creator), Petela, N. J. (Creator), Scheinost, J. C. (Creator), Collier, J. (Creator), Voulgaris, M. (Creator), Roig, M. B. (Creator), Beckouët, F. (Creator), Hu, B. (Creator) & Nasmyth, K. N. (Creator), GEO, 6 Jun 2019
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE132221
Dataset

Bin Hu
Person: Academic

Cite this

@article{3e3126e752554493893ac3042b7f21ad,

title = "Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1",

abstract = "Cohesin{\textquoteright}s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1{\textquoteright}s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin{\textquoteright}s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.",

author = "Madhusudhan Srinivasan and Petela, {Naomi J} and Scheinost, {Johanna C} and James Collier and Menelaos Voulgaris and Roig, {Maurici B} and Frederic Beckou{\"e}t and Bin Hu and Nasmyth, {Kim A}",

note = "Acknowledgements Maria Demidova conducted initial experiments that this study expanded on. We are grateful to Tomo Tanaka and Seiji Tanaka for supplying reagents. We thank all members of the Nasmyth group for valuable discussions, technical assistance and critical reading of the manuscript. This work was funded by the Wellcome Trust Senior Investigator Award, Grant Ref 107935/Z/15/Z and Cancer Research UK Programme Grant, Grant Ref 26747 to KN. BH is funded by (202062/Z/16/Z).",

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AU - Srinivasan, Madhusudhan

AU - Petela, Naomi J

AU - Scheinost, Johanna C

AU - Collier, James

AU - Voulgaris, Menelaos

AU - Roig, Maurici B

AU - Beckouët, Frederic

AU - Hu, Bin

AU - Nasmyth, Kim A

N1 - Acknowledgements Maria Demidova conducted initial experiments that this study expanded on. We are grateful to Tomo Tanaka and Seiji Tanaka for supplying reagents. We thank all members of the Nasmyth group for valuable discussions, technical assistance and critical reading of the manuscript. This work was funded by the Wellcome Trust Senior Investigator Award, Grant Ref 107935/Z/15/Z and Cancer Research UK Programme Grant, Grant Ref 26747 to KN. BH is funded by (202062/Z/16/Z).

PY - 2019/6/21

Y1 - 2019/6/21

N2 - Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.

AB - Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.

U2 - 10.7554/elife.44736

DO - 10.7554/elife.44736

M3 - Article

VL - 8

JO - eLife

JF - eLife

M1 - e44736

ER -

Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1

Abstract

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Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1

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