Unsolved mysteries of Rag GTPase signaling in yeast

Riko Hatakeyama, Claudio De Virgilio* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


The target of rapamycin complex 1 (TORC1) plays a central role in controlling eukaryotic cell growth by fine-tuning anabolic and catabolic processes to the nutritional status of organisms and individual cells. Amino acids represent essential and primordial signals that modulate TORC1 activity through the conserved Rag family GTPases. These assemble, as part of larger lysosomal/vacuolar membrane-associated complexes, into heterodimeric sub-complexes, which typically comprise two paralogous Rag GTPases of opposite GTP-/GDP-loading status. The TORC1-stimulating/inhibiting states of these heterodimers are controlled by various guanine nucleotide exchange factor (GEF) and GTPase-activating protein (GAP) complexes, which are remarkably conserved in various eukaryotic model systems. Among the latter, the budding yeast Saccharomyces cerevisiae has been instrumental for the elucidation of basic aspects of Rag GTPase regulation and function. Here, we discuss the current state of the respective research, focusing on the major unsolved issues regarding the architecture, regulation, and function of the Rag GTPase containing complexes in yeast. Decoding these mysteries will undoubtedly further shape our understanding of the conserved and divergent principles of nutrient signaling in eukaryotes.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalSmall GTPases
Issue number4
Early online date15 Aug 2016
Publication statusPublished - 2016


  • Amino Acids/metabolism
  • GTP Phosphohydrolases/metabolism
  • Gene Expression Regulation, Fungal
  • Saccharomyces cerevisiae/enzymology
  • Saccharomyces cerevisiae Proteins/metabolism
  • Signal Transduction
  • Transcription Factors/metabolism


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