VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain

Miguel Tillo; Lynda Erskine; Anna Cariboni; Alessandro Fantin; Andy Joyce; Laura Denti; Christiana Ruhrberg

doi:10.1242/dev.115998

VEGF₁₈₉ binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain

Miguel Tillo, Lynda Erskine, Anna Cariboni, Alessandro Fantin, Andy Joyce, Laura Denti, Christiana Ruhrberg

Medical Sciences

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

11 Downloads (Pure)

Abstract

The vascular endothelial growth factor (VEGFA, VEGF) regulates neurovascular patterning. Alternative splicing of the Vegfa gene gives rise to three major isoforms termed VEGF121, VEGF165 and VEGF189. VEGF165 binds the transmembrane protein neuropilin 1 (NRP1) and promotes the migration, survival and axon guidance of subsets of neurons, whereas VEGF121 cannot activate NRP1-dependent neuronal responses. By contrast, the role of VEGF189 in NRP1-mediated signalling pathways has not yet been examined. Here, we have combined expression studies and in situ ligand-binding assays with the analysis of genetically altered mice and in vitro models to demonstrate that VEGF189 can bind NRP1 and promote NRP1-dependent neuronal responses.

Original language	English
Pages (from-to)	314-319
Number of pages	6
Journal	Development
Volume	142
Issue number	2
Early online date	17 Dec 2014
DOIs	https://doi.org/10.1242/dev.115998
Publication status	Published - Jan 2015

Bibliographical note

© 2015. Published by The Company of Biologists Ltd.
This research was funded by a Wellcome Trust PhD fellowship to M.T. [092839/Z/10/Z] and a BBSRC project grant to C.R. and L.E. [BB/J00930X/1]. Deposited in PMC for immediate release.

Keywords

vascular endothelial growth factor (VEGF)
VEGF189
neuron
neuropilin
mouse

Access to Document

10.1242/dev.115998Licence: CC BY

VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Final published version, 881 KBLicence: CC BY

Lynda Erskine
- School of Medicine, Medical Sciences & Nutrition, Molecular and Cellular Function
- School of Medicine, Medical Sciences & Nutrition, Medical Sciences - Chair in Development Neurobiology
- School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences
Person: Academic

Cite this

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title = "VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain",

abstract = "The vascular endothelial growth factor (VEGFA, VEGF) regulates neurovascular patterning. Alternative splicing of the Vegfa gene gives rise to three major isoforms termed VEGF121, VEGF165 and VEGF189. VEGF165 binds the transmembrane protein neuropilin 1 (NRP1) and promotes the migration, survival and axon guidance of subsets of neurons, whereas VEGF121 cannot activate NRP1-dependent neuronal responses. By contrast, the role of VEGF189 in NRP1-mediated signalling pathways has not yet been examined. Here, we have combined expression studies and in situ ligand-binding assays with the analysis of genetically altered mice and in vitro models to demonstrate that VEGF189 can bind NRP1 and promote NRP1-dependent neuronal responses.",

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AU - Tillo, Miguel

AU - Erskine, Lynda

AU - Cariboni, Anna

AU - Fantin, Alessandro

AU - Joyce, Andy

AU - Denti, Laura

AU - Ruhrberg, Christiana

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