Inflammation is central in disease pathophysiology and accurate methods for its detection and quantification are increasingly required to guide diagnosis and therapy. Here we explored the ability of Fast Field-Cycling Magnetic Resonance (FFC-MR) in quantifying the signal of ultra-small superparamagnetic iron oxide particles (USPIO) phagocytosed by J774 macrophage-like cells as a proof-of-principle.
Relaxation rates were measured in suspensions of J774 macrophage-like cells loaded with USPIO (0-200μg/ml Fe as ferumoxytol), using a 0.25 T FFC benchtop relaxometer and a human whole-body, in-house built 0.2 T FFC-MR prototype system with a custom test tube coil. Identical non-imaging, saturation recovery pulse sequence with 90o flip angle and 20 different evolution fields selected logarithmically between 80 μT and 0.2T (3.4kHz and 8.51 MHz proton Larmor frequency [PLF] respectively). Results were compared with imaging flow cytometry quantification of side scatter intensity and USPIO-occupied cell area. A reference colorimetric iron assay was used.
The T1 dispersion curves derived from FFC-MR were excellent in detecting USPIO at all concentrations examined (0-200μg/ml Fe as ferumoxytol) vs. control cells, p ≤ 0.001. FFC-NMR was capable of reliably detecting cellular iron content as low as 1.12ng/µg cell protein, validated using a colorimetric assay. FFC-MR was comparable to imaging flow cytometry quantification of side scatter intensity but superior to USPIO-occupied cell area, the latter being only sensitive at exposures ≥ 10µg/ml USPIO.
We demonstrated for the first time that FFC-MR is capable of quantitative assessment of intra-cellular iron which will have important implications for the use of USPIO in a variety of biological applications, including the study of inflammation.
Bibliographical noteDKD would like to declare funding from British Heart Foundation Project Grant PG/15/108/31928 with no financial conflict of interest. DJL would like to declare funding from the European Commission – ‘Improving Diagnosis by Fast Field-Cycling MRI’ grant number 668119 with no financial conflict of interest, and GE Healthcare in the form of funding for PhD studentship in radiofrequency coils for FFC-MRI, with potential financial conflict of interest. The authors have no additional financial interests.
- fast field-cycling magnetic resonance
- ultrasmall superparamagnetic iron oxide particles (USPIO)
- Fast field-cycling magnetic resonance
- Ultrasmall superparamagnetic iron oxide particles (USPIO)
- CONTRAST AGENTS
- SUPERPARAMAGNETIC PARTICLES
FingerprintDive into the research topics of 'Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept'. Together they form a unique fingerprint.
Andrea Holme (Manager)Iain Fraser Cytometry Centre
Research Facilities: Equipment